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Protein Transport to the Stroma

Protein Transport to the Stroma

Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Protein Transport to the Outer Chloroplast Membrane

Protein Transport to the Outer Chloroplast Membrane

Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.

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Updated: Jun 23, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on ¹⁵N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

Chlamydomonas proteomics.

Norbert Rolland¹, Ariane Atteia, Paulette Decottignies

¹Laboratoire de Physiologie Cellulaire Végétale, CNRS, INRA, Université Joseph Fourier, CEA, Grenoble, France. norbert.rolland@cea.fr

Current Opinion in Microbiology

|May 20, 2009

Summary

This summary is machine-generated.

Proteomics studies in the model organism Chlamydomonas reinhardtii reveal key insights into photosynthesis, molecular biology, and evolution. This review covers recent technical and biological advancements in Chlamydomonas proteomics research.

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Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

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A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

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Published on: March 13, 2014

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

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Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

Area of Science:

* Biochemistry and Molecular Biology
* Evolutionary Biology
* Photosynthesis Research

Background:

* Chlamydomonas reinhardtii, a unicellular alga, uniquely combines plant and animal characteristics.
* Its simplicity and genetic tractability make it an ideal model organism.
* Proteomics studies have significantly advanced understanding in photosynthesis, molecular biology, and evolution.

Purpose of the Study:

* To review recent technical and biological aspects of proteomics studies in Chlamydomonas reinhardtii.
* To highlight the contributions of Chlamydomonas proteomics to various scientific fields.

Main Methods:

* Analysis of proteomics-based studies on Chlamydomonas reinhardtii.
* Focus on technical advancements and biological applications in recent research.

Main Results:

* Proteomics has yielded substantial contributions to the understanding of photosynthesis.
* Significant progress has been made in Chlamydomonas molecular biology and evolutionary studies.
* Recent research showcases innovative approaches in Chlamydomonas proteomics.

Conclusions:

* Chlamydomonas reinhardtii remains a powerful model organism for diverse biological investigations.
* Ongoing proteomics research continues to deepen our knowledge of fundamental biological processes.
* The review underscores the value of Chlamydomonas proteomics for future discoveries.