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Updated: Jun 23, 2026
Isolation of Human Primary Valve Cells for In vitro Disease Modeling
Published on: April 16, 2021
A technique for in vitro culture of canine valvular interstitial cells
Allison M Heaney1, Barret J Bulmer, Christopher R Ross
1Department of Clinical Sciences, Kansas State University, College of Veterinary Medicine, Manhattan, KS 66506, USA. aheaney@vetmed.wsu.edu
Objective:
To develop a method for in vitro culture of canine valvular interstitial cells (VICs).
Animals, Materials And Methods:
Canine VICs were isolated from the distal third of the anterior mitral valve leaflet using an explant technique and maintained in cell culture. Molecular phenotyping of the cultured cells was performed using reverse transcription polymerase chain reaction and immunocytochemistry.
Results:
Cells resembling fibroblasts migrated from canine mitral valve explants and were maintained in culture for up to eight passages. Establishment of the valve explant required collagen but once established, subsequent passages grew on non-coated plastic plates. At confluence the cultured cells exhibited the characteristic whorled pattern of fibroblasts in culture. The isolated valve cells expressed vimentin but not platelet endothelial cell adhesion molecule or von Willebrand's factor, consistent with the molecular phenotype of VICs.
Conclusions:
VICs can be readily isolated from canine mitral valve leaflets and successfully maintained in culture using standard culture techniques. The described techniques permit the study of bioactive VICs in a controlled environment and may be a useful in vitro model for investigation of cellular and molecular alterations associated with canine chronic degenerative valve disease.
