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Related Experiment Video

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A Modified Surgical Model of Hind Limb Ischemia in ApoE-/- Mice using a Miniature Incision
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Microparticles from ischemic muscle promotes postnatal vasculogenesis.

Aurelie S Leroyer1, Téni G Ebrahimian, Clément Cochain

  • 1Paris Cardiovascular Research Center, INSERM U, Hôpital Européen Georges Pompidou, Université Paris-Descartes, France.

Circulation
|May 20, 2009
PubMed
Summary

Microparticles released during tissue ischemia promote new blood vessel formation by stimulating progenitor cell differentiation. These findings highlight the role of microparticles in post-ischemic vasculogenesis.

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Area of Science:

  • Biomedical Science
  • Cell Biology
  • Vascular Biology

Background:

  • Microparticles (MPs) are released during tissue ischemia.
  • These MPs are hypothesized to be endogenous signals driving post-ischemic vasculogenesis.

Purpose of the Study:

  • To investigate the role of microparticles in post-ischemic vasculogenesis.
  • To determine if MPs from ischemic tissue stimulate progenitor cell differentiation and promote revascularization.

Main Methods:

  • MPs were isolated from ischemic mouse hind-limb muscle using sequential centrifugation.
  • Flow cytometry and electron microscopy were used for MP characterization.
  • In vitro assays assessed MP-induced bone marrow-mononuclear cell (BM-MNC) differentiation.
  • In vivo studies evaluated the effect of MPs on post-ischemic revascularization in a mouse hind-limb model.

Main Results:

  • MPs from ischemic muscle were significantly increased and primarily derived from endothelial cells.
  • Ischemic MPs enhanced in vitro BM-MNC differentiation into endothelial cells.
  • MPs from ischemic muscle showed increased expression of NADPH oxidase subunits (p47, p67).
  • MP injection improved BM-MNC-mediated revascularization in ischemic hind limbs, an effect dependent on gp91 expression.

Conclusions:

  • Microparticles generated during tissue ischemia are potent stimulators of progenitor cell differentiation.
  • These MPs play a crucial role in promoting postnatal neovascularization after ischemia.
  • The findings elucidate a novel mechanism for endogenous repair following ischemic events.