Related Experiment Video
Updated: Jun 23, 2026

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
Published on: May 10, 2024
P-selectin inhibition reduces severity of acute experimental pancreatitis
Thilo Hackert1, Rasmus Sperber, Werner Hartwig
1Department of Surgery and Experimental Surgery, University of Heidelberg, Heidelberg, Germany. thilo_hackert@med.uni-heidelberg.de
Background:
Acute pancreatitis (AP) is characterized by disturbed pancreatic microcirculation with tissue necrosis. Platelet and leukocyte activation contributes to microcirculatory disorders and inflammatory tissue infiltration. P-selectin mediates the adhesion of both activated platelets and leukocytes to the vessel wall. The aim of this study was to investigate the effect of P-selectin inhibition by monoclonal antibodies (AB) on experimental AP.
Methods:
AP was induced in rats by glycodeoxycholic acid (GDOC) intraductally and by cerulein infusion. Animals were divided into 4 groups: (1) severe AP (GDOC); (2) severe AP + platelet inhibition (GDOC + selectin AB); (3) control (Ringer); (4) control + platelet inhibition (Ringer + selectin AB). 24 h after AP induction, histology and serum (amylase, thromboxane A2) were evaluated (6 animals per group). In additional 12 animals of each group, platelet and leukocyte activation as well as erythrocyte flow patterns were evaluated by intravital microscopy 12 h after AP induction.
Results:
AP induction caused significant tissue inflammation and necrosis with increased amylase and thromboxane levels. Prophylactic inhibition of P-selectin reduced tissue inflammation and necrosis significantly. Severe AP led to significantly more adherent platelets and leukocytes in capillaries and venules. In contrast, antibody-treated animals showed significantly reduced platelet-endothelium interaction compared with untreated AP animals. Antibody application in control animals without AP did not induce any changes compared with healthy control animals.
Conclusion:
Inhibition of P-selectin reduces tissue damage in experimental AP. This is associated with a reduction in platelet- and leukocyte-endothelium interaction and an improvement in pancreatic microcirculation.
Related Concept Videos
Acute Pancreatitis II: Pathophysiology
Acute Pancreatitis I: Introduction
Acute Pancreatitis I: Introduction
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
Acute Pancreatitis II: Clinical Manifestations and Management
Chronic Pancreatitis II: Pathophysiology
Chronic Pancreatitis I: Introduction

