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Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

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Related Experiment Video

Updated: Jun 23, 2026

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Published on: February 28, 2014

Zebrafish wnt3 is expressed in developing neural tissue.

Wilson K Clements¹, Karen G Ong, David Traver

¹Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093-0380, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|May 20, 2009

Summary

Zebrafish wnt3 is expressed in neural tissues, suggesting roles in brain development, but not early mesoderm formation. This allows future studies on later Wnt3-regulated neural patterning via knockdown.

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Last Updated: Jun 23, 2026

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High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

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Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

Published on: August 4, 2014

Area of Science:

Developmental Biology
Genetics
Neuroscience

Background:

Wnt signaling is crucial for embryonic development and stem cell maintenance.
Wnt3 plays key roles in mouse embryonic patterning, including mesoderm specification and neural development.
Aberrant Wnt signaling is implicated in various diseases.

Purpose of the Study:

To identify and characterize the zebrafish wnt3 gene.
To investigate the expression patterns of wnt3 during zebrafish embryonic development.
To determine the potential role of Wnt3 in zebrafish mesoderm and neural patterning.

Main Methods:

Identification and preliminary characterization of the zebrafish wnt3 gene.
Whole-mount in situ hybridization to analyze wnt3 expression patterns in zebrafish embryos.

Main Results:

Zebrafish wnt3 is expressed in the developing tailbud and specific neural tissues, including the ZLI, optic tectum, midbrain-hindbrain boundary, and dorsal hindbrain/spinal cord.
Contrary to mouse studies, wnt3 expression was not detected during zebrafish mesoderm specification.
The expression pattern suggests Wnt3 involvement in forebrain compartmentalization and neural patterning.

Conclusions:

Zebrafish Wnt3 is dynamically expressed in neural tissues, indicating a role in central nervous system development.
The absence of early wnt3 expression in zebrafish challenges its role in mesoderm specification, unlike in mouse.
The expression profile supports the use of zebrafish wnt3 knockdown for studying later neural patterning events.