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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
Cloning and characterization of small non-coding RNAs from grape
Andrea Carra1, Erica Mica, Giorgio Gambino
1Department of Arboriculture and Pomology, University of Torino, Italy. andrea.m.carra@gmail.com
The Plant Journal : for Cell and Molecular Biology
|May 21, 2009
Summary
Grapevine small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), regulate fruit ripening and defense. These small RNAs target genes involved in plant development and stress responses, expanding known regulatory roles.
Area of Science:
- Plant Molecular Biology
- Genomics
- Biochemistry
Background:
- Small non-coding RNAs (miRNAs, siRNAs) are crucial regulators of plant processes.
- Understanding these regulators in grapevine (Vitis vinifera L.) is vital for fruit development and stress response insights.
Purpose of the Study:
- To characterize the nature and functions of small RNAs in grapevine berries.
- To identify miRNA and siRNA targets involved in plant development and stress.
Main Methods:
- Small RNA library construction from mixed-stage grape berries.
- Bioinformatic analysis for miRNA and siRNA identification and target prediction.
- Experimental validation of miRNA and siRNA targets using techniques like 5' RACE.
Main Results:
- Isolated 13 conserved miRNAs, 1 non-conserved miRNA, and 4 putative non-conserved miRNAs.
- Identified siRNA targets including genes for NB-LRR proteins, heavy metal transport, RD22-like proteins, and cytokinin synthase.
- Demonstrated siRNA id65 specifically represses cytokinin synthase during berry maturation, involving transcript processing and secondary siRNA action.
Conclusions:
- Grapevine miRNA and siRNA regulatory circuits are involved in defense and fruit ripening.
- Small RNA regulation in grapevine extends beyond known hormone pathways to include cytokinin metabolism.
- These findings broaden the scope of small RNA-mediated regulation in plants.
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