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Updated: Jun 23, 2026

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Published on: January 20, 2014
Reduction of sample evaporation in small volume microplate luminescence assays
1Sci-Tec Inc., Knoxville, TN 37932, USA. klvngrgry@hotmail.com
Abstract:
Luminescence detection has great potential for use in small volume assays for high-throughput applications. However, the need for automated injection of detection reagents dictates an open well format, making small volume luminescence assays very susceptible to sample evaporation effects. Here we describe a technique for reducing evaporation of small sample volumes that uses layering of silicone oil on solution surfaces but still allows the use of automated injection.
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