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Related Experiment Video

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A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Glycan reductive isotope labeling for quantitative glycomics.

Baoyun Xia1, Christa L Feasley, Goverdhan P Sachdev

  • 1Department of Biochemistry and Glycomics Center at Emory University, Emory University School of Medicine, Atlanta, GA 30322, USA.

Analytical Biochemistry
|May 21, 2009
PubMed
Summary

A new glycomics method, glycan reductive isotope labeling (GRIL), enables precise quantification of complex carbohydrates. This technique advances the study of diseases linked to glycan changes.

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Last Updated: Jun 23, 2026

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Complex carbohydrates (glycans) play crucial roles in biological processes.
  • Alterations in glycan structure and quantity are hallmarks of various diseases and disorders.
  • Accurate glycomics analysis is essential for understanding disease mechanisms and developing diagnostics.

Purpose of the Study:

  • To introduce and validate a novel glycomics method, glycan reductive isotope labeling (GRIL).
  • To enable sensitive and quantitative analysis of free and released glycans.
  • To provide a robust tool for comparative glycomics studies.

Main Methods:

  • GRIL involves derivatizing free glycans via reductive amination using stable isotope-coded aniline tags ([(12)C(6)]aniline and [(13)C(6)]aniline).
  • Aniline-tagged glycans are purified using reverse-phase chromatography and quantified via UV absorbance and mass spectrometry.
  • The method avoids deuterium labeling, preventing chromatographic separation of differentially tagged glycans.

Main Results:

  • GRIL allows direct comparison and quantification of differentially labeled glycan mixtures using mass spectrometry.
  • The method demonstrated linear relative quantitation of glycans over a 10-fold concentration range.
  • GRIL accurately quantifies sub-picomole levels of released glycans from various sources, including serum glycoproteins.

Conclusions:

  • GRIL is a sensitive and reliable method for quantitative glycomics.
  • This technique offers significant advantages over existing isotopic labeling methods for glycans.
  • GRIL represents a valuable advancement for glycomics research, particularly in disease biomarker discovery.