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Updated: Jun 23, 2026
Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells
Published on: May 20, 2015
Automated soft agar assay for the high-throughput screening of anticancer compounds
René Thierbach1, Pablo Steinberg
1Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany. rene.thierbach@tiho-hannover.de
Abstract:
We have established an automated soft agar colony formation assay that can be used as a potent tool in experimental tumor therapy studies as well as anticancer compound screening. It allows the direct and simultaneous comparison of the effects of a high number of anticancer compounds on the anchorage-independent growth of a variety of tumor cell lines. By making use of a commercially available automated pipetting system, the user gets results of excellent quality within 1 week and does not need special cell culture practice.
