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Hydrogen Bonds

Hydrogen Bonds

Hydrogen BondsHydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.Hydrogen Bonds Control the World!Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are...

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Related Experiment Video

Updated: Jun 23, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Protein Film Infrared Electrochemistry Demonstrated for Study of H₂ Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

Dynamic electrochemical experiments on hydrogenases.

Fraser A Armstrong¹

¹Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, Oxford OX1 3QR, UK. fraser.armstrong@chem.ox.ac.uk

Photosynthesis Research

|May 21, 2009

Summary

This summary is machine-generated.

Protein film electrochemistry offers new insights into hydrogenase enzymes. This dynamic technique reveals enzyme kinetics, catalytic bias, and responses to oxygen, crucial for hydrogen production and energy generation.

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Last Updated: Jun 23, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

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Area of Science:

Biochemistry
Electrochemistry
Enzymology

Background:

Hydrogenases are crucial enzymes involved in hydrogen metabolism.
Understanding their kinetics and inhibition is vital for biotechnological applications.
Current methods provide limited quantitative data on enzyme function.

Purpose of the Study:

To apply protein film electrochemistry for detailed analysis of hydrogenases.
To quantify hydrogenase kinetics, catalytic bias, and oxygen tolerance.
To explore adaptations for photosynthetic hydrogen production and aerobic energy generation.

Main Methods:

Utilized dynamic electrochemical techniques, specifically protein film electrochemistry.
Studied kinetics, catalytic bias (H2 oxidation vs. H2 production), and inhibitor responses.
Quantified enzyme behavior using minimal enzyme quantities.

Main Results:

Generated extensive quantitative data on hydrogenase function.
Revealed differences in oxygen and inhibitor reactivity.
Established kinetic parameters and catalytic preferences.

Conclusions:

Protein film electrochemistry is a powerful tool for studying hydrogenases.
Findings are critical for optimizing hydrogenase activity in biological systems.
The methodology can be adapted for other complex enzyme systems.