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Cloning and expression analysis of two opsin-like genes in the phytopathogenic fungus Bipolaris oryzae

Junichi Kihara1, Nozomi Tanaka, Makoto Ueno

  • 1Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan. j-kihara@life.shimane-u.ac.jp

FEMS Microbiology Letters
|May 22, 2009
PubMed

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Epistasis Analysis

Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...