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Related Experiment Video

Updated: Jun 23, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

C Schmidt1, C Camus, H Caudiu

  • 1Service de Biophysique, Recherche Appliquée et Développement L'Oreal, 188 rue Paul Hochart, 94550 Chevilly-Larue, France.

International Journal of Cosmetic Science
|May 22, 2009
PubMed
Summary

Two methods for measuring skin relief, profilometry and image analysis, show strong correlations. Image analysis wrinkle depth is influenced by surface area and peak height, particularly for medium wrinkles.

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Area of Science:

  • Dermatology
  • Biomedical Engineering
  • Image Analysis

Background:

  • Cutaneous relief measurement is crucial for understanding skin aging and texture.
  • Existing methods like profilometry and image analysis have different approaches to quantifying skin topography.

Purpose of the Study:

  • To correlate the results of profilometry and image analysis for measuring cutaneous relief.
  • To determine the relationship between different parameters measured by each technique.

Main Methods:

  • Correlating profilometry (measuring skin-positive araldite replicas) and image analysis (analyzing silicone replicas with glazing light).
  • Analyzing twenty-nine crow's feet replicas using defined scan areas and intervals (D 60 µm and D 900 µm).
  • Assessing parameters like peak height (H), surface area under peaks (S), wrinkle depth (P), and coefficient of developed skin surface (CDSS).

Related Experiment Videos

Last Updated: Jun 23, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

Main Results:

  • The best correlations between image analysis and profilometry were observed for the D 900 µm interval.
  • Image analysis wrinkle depth (P) was significantly influenced by surface area (S) (46%) and profilometry peak height (H) (33.5%).
  • The coefficient of developed skin surface (CDSS) was influenced by H (45%) and S (44%).

Conclusions:

  • Profilometry and image analysis are comparable methods for quantifying skin relief, especially for medium wrinkles (45-100 µm depth).
  • The D 900 µm interval in profilometry provides results that correlate well with image analysis findings.
  • Understanding the interplay between surface area and peak height is key to accurate skin topography assessment.