Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High KIR diversity in Uganda and Botswana children living with HIV.

Human immunology·2026
Same author

Association of Human Cytomegalovirus exposure with tuberculosis disease in South African adults with presumptive tuberculosis.

PLOS global public health·2026
Same author

Conserved neutrophil degranulation transcripts in HIV-TB coinfected children across East and Southern Africa.

Communications medicine·2026
Same author

Patterns of compensatory mutations in rpoA/B/C genes of multidrug resistant M. tuberculosis in Uganda.

PloS one·2025
Same author

PERFORMANCE OF FUJILAM VERSION 1 FOR THE DIAGNOSIS OF TUBERCULOSIS IN CHILDREN UNDER 5 YEARS IN UGANDA - A BRIEF REPORT.

medRxiv : the preprint server for health sciences·2025
Same author

Multidrug resistant hypervirulent ST307 clone from genomic surveillance of extended spectrum beta-lactamase-producing Klebsiella pneumoniae species complex in East Africa.

BMC microbiology·2025

Related Experiment Video

Updated: Jun 23, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
08:10

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Direct susceptibility testing for multi drug resistant tuberculosis: a meta-analysis.

Freddie Bwanga1, Sven Hoffner, Melles Haile

  • 1Department of Medical Microbiology Makerere University College of Health Sciences Kampala, Uganda. freddie.bwanga@ki.se

BMC Infectious Diseases
|May 22, 2009
PubMed
Summary

Rapid molecular and phenotypic tests accurately detect multidrug-resistant tuberculosis (MDR TB) directly from sputum samples. These faster methods aid timely patient management in resource-limited settings.

Related Experiment Videos

Last Updated: Jun 23, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
08:10

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • Tuberculosis (TB) control programs face challenges in resource-limited settings due to the lack of rapid drug-resistant TB detection methods.
  • Conventional indirect susceptibility testing yields results too late for timely patient management.
  • There is a need for rapid tests directly applicable to sputum samples for detecting drug resistance.

Purpose of the Study:

  • To compare the sensitivity, specificity, and time to results of four direct drug susceptibility testing (DST) methods.
  • To evaluate two in-house phenotypic assays (NRA, MODS) and two commercial molecular assays (Genotype MTBDR, Genotype MTBDRplus).
  • To assess these direct DST methods against conventional indirect testing for rifampicin and isoniazid resistance in Mycobacterium tuberculosis.

Main Methods:

  • A literature review and meta-analysis of 18 direct DST reports.
  • Analysis using Meta-Disc software to determine sensitivity, specificity, and area under the sROC curves.
  • Heterogeneity testing using Spearman correlation coefficient and Chi-square.

Main Results:

  • All four direct DST methods demonstrated high pooled sensitivity and specificity for rifampicin and isoniazid resistance.
  • NRA and Genotype MTBDRplus showed >99% sensitivity and >98% specificity for rifampicin resistance.
  • Molecular tests (Genotype MTBDR/MTBDRplus) provided results in 1-2 days, significantly faster than conventional methods.

Conclusions:

  • Direct drug susceptibility testing for rifampicin and isoniazid resistance in M. tuberculosis is highly sensitive and specific.
  • These rapid diagnostic methods allow for prompt detection of multidrug-resistant tuberculosis (MDR TB).
  • The findings support the implementation of rapid DST in resource-limited settings for improved TB control.