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An inexpensive inverted microscope for patch-clamp and other electrophysiological studies at the cellular level
1Department of Physiology, Faculty of Medicine, University of Ottawa, Ottawa Civic Hospital, Ontario, Canada.
Pflugers Archiv : European Journal of Physiology
|July 1, 1991
Summary
This study details an inexpensive, adaptable inverted microscope designed for patch-clamp electrophysiology. It offers unrestricted pipette movement, overcoming limitations of commercial models for cellular research.
Area of Science:
- Biophysics
- Cellular Neuroscience
- Microscopy
Background:
- The patch-clamp technique requires specialized inverted microscopes for optimal pipette maneuverability.
- Commercial inverted microscopes often lack sufficient space for patch-clamp apparatus, restricting pipette movement.
Purpose of the Study:
- To design and construct an affordable inverted microscope tailored for patch-clamp and electrophysiological investigations.
- To provide a versatile platform that facilitates unhindered microtool manipulation.
Main Methods:
- Detailed construction plans for a cost-effective inverted microscope.
- Design considerations prioritizing free movement of patch-clamp pipettes and associated equipment.
- Adaptability for various electrophysiological setups and micromanipulator probes.
Main Results:
- A functional inverted microscope meeting patch-clamp specifications was successfully built.
- The microscope design allows for unrestricted movement of patch-clamp pipettes and other microtools.
- The instrument is relatively inexpensive and easily customizable for advanced applications.
Conclusions:
- The developed inverted microscope effectively addresses the spatial limitations of commercial models for patch-clamp studies.
- This cost-effective and adaptable instrument is suitable for cellular electrophysiology and other micro-manipulation-intensive research.
- The design offers a valuable alternative for researchers requiring enhanced microtool access in microscopy.