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Updated: Jun 23, 2026
03:53
Extracellular Vesicle Tissue Factor Activity Assay
Published on: December 29, 2023
Tissue factor and factor v involvement in rat peritoneal fibrosis
Hiroki Saito1, Masayuki Kitamoto, Kozue Kato
1Division of Molecular Medicine, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan.
Summary
Tissue factor and factor V expression in macrophages contribute to peritoneal fibrosis by promoting blood vessel growth and extracellular matrix buildup. Inhibiting factor Xa may reduce these fibrotic changes.
Area of Science:
- Nephrology
- Pathology
- Biochemistry
Background:
- Long-term peritoneal dialysis can lead to fibrin deposition and peritoneal fibrosis.
- The roles of tissue factor and factor V in this process are not fully understood.
Purpose of the Study:
- To investigate the contribution of tissue factor and factor V to peritoneal fibrosis.
- To explore the potential of factor Xa inhibition as a therapeutic strategy.
Main Methods:
- Wistar rats received daily intraperitoneal injections of chlorhexidine gluconate (CG) for 28 days.
- A factor Xa inhibitor, fondaparinux, was administered subcutaneously in a subset of rats.
- Peritoneal tissues were analyzed using immunohistochemistry and in situ hybridization.
Main Results:
- CG injection significantly thickened the peritoneal submesothelial compact zone in a dose-dependent manner.
- Massive deposition of fibrin, fibronectin, and type IV collagen was observed in CG-injected rats.
- Intense staining for tissue factor, factor V, factor X, and protease-activated receptor-2 was noted in macrophages within the fibrotic peritoneum.
- Fondaparinux treatment reduced peritoneal fibrosis and angiogenesis.
Conclusions:
- Tissue factor and factor V expression in macrophages, along with factor X deposition, likely drive peritoneal fibrosis.
- These factors may promote angiogenesis and extracellular matrix accumulation through profibrotic and procoagulant mechanisms.
- Factor Xa inhibition shows promise in mitigating peritoneal fibrosis.
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