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Updated: Aug 3, 2026
12:34
DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
Human Alu subfamilies and their methylation revealed by blot hybridization
1Department of Genetics, University of California, Davis 95616.
Nucleic Acids Research
|October 25, 1991
Abstract:
By a simple direct blot hybridization strategy, the existence of human Alu family subfamilies is confirmed. Using consensus restriction cleavage sites, individual bands can be resolved from genomic human DNA digests corresponding to three distinct Alu subfamilies. Digestion with methylation sensitive and insensitive restriction enzymes shows that the numerous CpG residues in the youngest Alu subfamilies are largely methylated in vivo, suggesting a model for the transcriptional regulation of Alu repeats.
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