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A Multimodal Wide-Field Fourier-Transform Raman Microscope
Published on: December 30, 2025
Microsystem 671 nm light source for shifted excitation Raman difference spectroscopy
Martin Maiwald1, Heinar Schmidt, Bernd Sumpf
1Ferdinand-Braun-Institut für Höchstfrequenztechnik, Gustav-Kirchhoff-Strasse 4, D-12489 Berlin, Germany. Martin.Maiwald@FBH-Berlin.de
Applied Optics
|May 22, 2009
Summary
A new compact diode laser system provides stable, shifted excitation for Raman spectroscopy. This system effectively minimizes fluorescence interference in shifted excitation Raman difference spectroscopy (SERDS) applications.
Area of Science:
- Optics and Photonics
- Spectroscopy
- Laser Technology
Background:
- Shifted Excitation Raman Difference Spectroscopy (SERDS) is a powerful technique for analyzing samples with fluorescence.
- Developing a stable, compact light source is crucial for advancing SERDS applications.
- Existing laser systems may lack the necessary stability or spectral characteristics for optimal SERDS performance.
Purpose of the Study:
- To develop and characterize a compact, wavelength-stabilized diode laser system for SERDS.
- To evaluate the performance of the laser system in suppressing fluorescence in SERDS.
- To demonstrate the suitability of the laser for analyzing complex samples.
Main Methods:
- The laser system utilizes two broad-area gain media in separate cavities with Bragg gratings.
- Wavelength stabilization is achieved through precise control of cavity parameters.
- The system's performance was tested using ethanol samples with varying concentrations of Cresyl Violet as a fluorescent contaminant.
Main Results:
- A spectral width below 100 pm was achieved.
- A constant wavelength shift of 0.57 ± 0.06 nm was maintained up to 250 mW output power.
- Successful demonstration of SERDS with reduced fluorescence interference was achieved.
Conclusions:
- The developed compact diode laser system is a suitable light source for SERDS.
- The system offers excellent wavelength stability and a precise spectral shift.
- This technology has the potential to enhance the analysis of fluorescent samples using Raman spectroscopy.
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