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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 23, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic

Xiaole Mao1, Sz-Chin Steven Lin, Cheng Dong

  • 1Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.

Lab on a Chip
|May 22, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces microfluidic drifting for 3D hydrodynamic focusing in flow cytometry. This technique enables high-throughput cell analysis on a single-layer microfluidic chip.

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Last Updated: Jun 23, 2026

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Analytical Chemistry

Background:

  • Traditional flow cytometry often requires complex instrumentation.
  • Achieving precise 3D hydrodynamic focusing in planar microfluidic devices presents a challenge.
  • Efficient cell focusing is crucial for high-throughput analysis.

Purpose of the Study:

  • To develop a novel 3D hydrodynamic focusing technique for microfluidic flow cytometry.
  • To integrate this technique into a functional flow cytometry platform.
  • To demonstrate high-throughput cell analysis capabilities.

Main Methods:

  • Utilizing microfluidic drifting, induced by Dean flow in a curved channel, for vertical cell focusing.
  • Implementing a single-layer planar microfluidic device for 3D hydrodynamic focusing.
  • Integrating the focusing device with a laser-induced fluorescence (LIF) detection system.

Main Results:

  • Microfluidic drifting effectively achieved 3D hydrodynamic focusing of microparticles comparable to human CD4+ T lymphocytes.
  • The developed flow cytometry platform demonstrated high-throughput measurements exceeding 1700 cells per second.
  • Theoretical calculations, numerical simulations, and experimental characterizations validated the technique's efficacy.

Conclusions:

  • Microfluidic drifting offers a robust method for 3D hydrodynamic focusing in microfluidic systems.
  • The integrated flow cytometry platform provides a high-throughput, on-chip solution for cell analysis.
  • This technology has potential applications in various cell-based assays and diagnostics.