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Updated: Jun 23, 2026
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Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration
Published on: June 9, 2023
[Grape seed extract inhibit proliferation of breast cancer cell MCF-7 and decrease the gene expression of survivin]
Changjie Chen1, Chenbiao Liu, Ju Zhang
1Bengbu Medical College, Bengbu 233030, China. bbmcccj@sohu.com
Summary
Grape seed extract (GSE) effectively inhibits breast cancer cell (MCF-7) proliferation by arresting the cell cycle. GSE also down-regulates survivin gene expression, offering a potential therapeutic mechanism.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Breast cancer remains a leading cause of mortality worldwide.
- Identifying novel therapeutic agents with minimal side effects is crucial.
- Grape seed extract (GSE) is a natural compound with reported antioxidant and anti-cancer properties.
Purpose of the Study:
- To evaluate the anti-proliferative effects of GSE on MCF-7 breast cancer cells.
- To investigate the impact of GSE on survivin gene expression.
- To elucidate the underlying molecular mechanisms of GSE's inhibitory action.
Main Methods:
- Cell proliferation was assessed using MTT assays.
- Cell cycle analysis was performed via flow cytometry.
- Survivin mRNA expression was quantified using RT-PCR.
- Core promoter activity of survivin was measured using a luciferase reporter assay.
- Bioinformatics tools were employed to analyze transcription factor binding sites.
Main Results:
- GSE demonstrated a dose- and time-dependent inhibition of MCF-7 cell proliferation.
- GSE treatment led to cell cycle arrest in the S phase.
- A significant down-regulation of survivin mRNA expression was observed.
- GSE significantly reduced the activity of the survivin core promoter.
Conclusions:
- GSE inhibits MCF-7 breast cancer cell proliferation by inducing S-phase cell cycle arrest.
- The mechanism involves the regulation of transcription factors associated with survivin core promoter activity.
- GSE's ability to decrease survivin gene expression presents a promising therapeutic strategy for breast cancer.
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