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[Grape seed extract inhibit proliferation of breast cancer cell MCF-7 and decrease the gene expression of survivin]

Changjie Chen1, Chenbiao Liu, Ju Zhang

  • 1Bengbu Medical College, Bengbu 233030, China. bbmcccj@sohu.com

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
|May 23, 2009
PubMed
Abstract

Insights

Grape seed extract (GSE) effectively inhibits breast cancer cell (MCF-7) proliferation by arresting the cell cycle. GSE also down-regulates survivin gene expression, offering a potential therapeutic mechanism.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Breast cancer remains a leading cause of mortality worldwide.
  • Identifying novel therapeutic agents with minimal side effects is crucial.
  • Grape seed extract (GSE) is a natural compound with reported antioxidant and anti-cancer properties.

Purpose of the Study:

  • To evaluate the anti-proliferative effects of GSE on MCF-7 breast cancer cells.
  • To investigate the impact of GSE on survivin gene expression.
  • To elucidate the underlying molecular mechanisms of GSE's inhibitory action.

Main Methods:

  • Cell proliferation was assessed using MTT assays.
  • Cell cycle analysis was performed via flow cytometry.
  • Survivin mRNA expression was quantified using RT-PCR.
  • Core promoter activity of survivin was measured using a luciferase reporter assay.
  • Bioinformatics tools were employed to analyze transcription factor binding sites.

Main Results:

  • GSE demonstrated a dose- and time-dependent inhibition of MCF-7 cell proliferation.
  • GSE treatment led to cell cycle arrest in the S phase.
  • A significant down-regulation of survivin mRNA expression was observed.
  • GSE significantly reduced the activity of the survivin core promoter.

Conclusions:

  • GSE inhibits MCF-7 breast cancer cell proliferation by inducing S-phase cell cycle arrest.
  • The mechanism involves the regulation of transcription factors associated with survivin core promoter activity.
  • GSE's ability to decrease survivin gene expression presents a promising therapeutic strategy for breast cancer.

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