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Updated: Jun 23, 2026
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
Published on: February 25, 2014
[Proliferation effect of neural stem cell of ginsenoside Rg1 in vitro]
Pengwei Zhuang1, Yanjun Zhang, Tan Pang
1Tianjin University of Traditional Chinese Medicine, Tianjin Laboratory of Chinese Medience Pharmacology, Tianjin 300193, China. zhuangpengwei@163.com
Objective:
To study the proliferation effect of neural stem cells (NSCs) of ginsenoside Rg1 in vitro.
Method:
NSCs from the embryonic rat (E14) were isolated, 10 mg x L(-1) BrdU were added in the medium. The NSCs were incubated together with 1, 10 micromol x L(-1) ginsenoside Rg1 for 48 hours, control group were setup. Then BrdU positive cell number were detected and counted by fluorescence microscop. The Hes1 and Mash1 mRNA expression were detected by real-time RT-PCR.
Result:
The number of BrdU positive cells in 10 micromol x L(-1) ginsenoside Rg1 group was increased significantly compared with the control group (40.5 +/- 10.9 vs 26.2 +/- 6.0, P < 0.01), and the Hes1 mRNA expression were increased significantly (1.00 +/- 0.11) vs (1.28 +/- 0.14), P < 0.05.
Conclusion:
ginsenoside Rg1 can promote the proliferation of neural stem cells in vitro, and this effect may be induced by the up-regulation Hes1 expression.
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