Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

Proteolytic enzyme engineering: a tool for wool.

Rita Araújo¹, Carla Silva, Raul Machado

¹Department of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, Portugal.

Biomacromolecules

|May 23, 2009

Summary

Researchers engineered a novel high molecular weight subtilisin by fusing Bacillus subtilis prosubtilisin E with an elastin-like polymer (ELP). This enzyme offers controlled wool finishing, reducing damage compared to commercial proteases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Expansion, functional diversification, and gene fusion events in the Ato protein family.

iScience·2026

Same author

Engineered chromogenic proteins with carbohydrate binding modules for advanced textile dyeing.

International journal of biological macromolecules·2026

Same author

Amino-functionalized bimetallic MOF nanozyme <i>via</i> solvent-assisted ligand exchange for interference-free phenolic detection.

Analytical methods : advancing methods and applications·2026

Same author

[Observational Study Protocol: Management of Anemia in Allogeneic Bone Marrow Donors].

Acta medica portuguesa·2026

Same author

Lignin-Based Nanoparticles Loaded with Essential Oils: Potential for Diabetic Foot Infection Management.

ACS materials Au·2026

Same author

Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?

Microbial cell factories·2026

Area of Science:

Biotechnology
Protein Engineering
Textile Chemistry

Background:

Enzyme engineering aims to optimize industrial bioprocesses by tailoring enzyme structure.
Current proteases for wool finishing cause excessive fiber damage due to uncontrolled diffusion.

Purpose of the Study:

To construct and characterize a novel high molecular weight subtilisin for controlled wool surface hydrolysis.
To evaluate the efficacy of the engineered enzyme in wool finishing applications.

Main Methods:

Gene fusion of Bacillus subtilis prosubtilisin E and an elastin-like polymer (ELP).
Biological production of the fusion protein in Escherichia coli.
Purification and application of the recombinant enzyme in wool finishing assays.

More Related Videos

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

Related Experiment Videos

Last Updated: Jun 23, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

Main Results:

The engineered subtilisinE-VPAVG(220) exhibited restricted activity to the wool cuticle.
Significant reductions in wool pilling, weight loss, and tensile strength loss were observed.
The novel enzyme overcomes limitations of unrestrained diffusion and fiber damage seen with commercial proteases.

Conclusions:

Microbial production of a functionalized high molecular weight protease for controlled wool hydrolysis is reported for the first time.
The engineered enzyme provides a more controlled and less damaging approach to wool finishing.
This protein engineering strategy offers a solution to the major obstacles in enzymatic wool treatment.