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Updated: Jun 23, 2026

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay
Published on: September 7, 2015
Biodegradation of the sulfonylurea herbicide chlorimuron-ethyl by the strain Pseudomonas sp. LW3
Ji-Ping Ma1, Zhe Wang, Peng Lu
1Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Abstract:
The chlorimuron-ethyl-degrading bacterium LW3 was isolated from contaminated soil and identified by 16S rRNA gene sequencing as Pseudomonas sp. When chlorimuron-ethyl was provided as the sole nitrogen source, the degradation efficiency in liquid medium was about 81.0% after 7 days of inoculation with strain LW3. The effects of chlorimuron-ethyl concentration and temperature on biodegradation were examined. Two metabolites of degradation were analyzed by LC/MS. Based on the identified products, strain LW3 seemed to be able to degrade chlorimuron-ethyl by cleavage of the sulfonylurea bridge. The inoculation of strain LW3 to chlorimuron-ethyl-treated soil resulted in a higher degradation rate than in uninoculated soil, regardless of the soil being sterilized or nonsterilized. This microbial culture has great potential for the bioremediation of soil contaminated with chlorimuron-ethyl.
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