Influence du butyrylhydroxyproline sur le developpement des fibroblastes en culture

Synopsis The action of butyrylhydroxyproline on the growth rate of fibroblasts in culture as well as their capacity to biosynthesize proteins has been demonstrated. The cellular cultures were carried out on a 'HAM'medium to which was added 10%, 5% or 2% of newborn calf serum. Cellular growth was estimated by numerical counting. The strains used came from human skin (fetus, child, adult) and were put in cell culture in cellular monolayer and used at similar states of subculture. Protein biosynthesis was studied through the incorporation of tritiated L-Proline or tritiated L-Leucine (5 muCi/ml of culture medium), either three days or eight days after reaching confluence. The examined products were: - Butyrylhydroxyproline in solution at 48%; - N-acetylhydroxyproline was used as reference product. After typing the cytotoxicity, the quantities incorporated were as follows: - 10 to 100 pg of pure product for each ml of culture medium for the butmlhydroxyproline; - 17.4 to 500 pg/ml for the N-acetylhydroxyproline. Whatever the applied conditions and the concentrations used, N-acetylhydroxyproline and butyrylhydroxyproline have no significant action on cellular growth. On the other hand, the authors showed an action of the above two products on the total protein bicp synthesis. Three to five days after reaching confluence, the lipacide and the N-acetyl- hydroxyproline provoked an increase (up to 120%) of the protein biosynthesis compared to the control. This action appeared when small doses were involved (10 to 20 pghol) and increased with concentration.