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Updated: Jun 23, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
Translocons on the inner and outer envelopes of chloroplasts share similar evolutionary origin in Arabidopsis
H-X Lv1, G Q Guo, Zhong-Nan Yang
1Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, China.
Abstract:
The process of importing nuclear encoded proteins into chloroplasts is mediated by the Translocons on the Outer/Inner Envelope of Chloroplasts (TOC and TIC complex). The ancestor of the TOC complex was formed by pre-existing proteins from the cyanobacterial ancestor; other proteins recruited from the host cell or cyanobacterial ancestor were subsequently integrated into the complex. However, little is known about the origin of the TIC complex. In this work, the origin of the TIC complex was investigated through one of its channel proteins, AtTic21. Phylogenetic analysis suggested that AtTic21 is conserved in photosynthetic organisms. AtTic21 showed 33% sequence identity to a Synechocystis protein SynTic21. The successful genetic complementation of an AtTic21 knockout mutant by SynTic21 plus the transit peptide coding sequence of AtTic21 suggested that SynTic21 is an ortholog of AtTic21. The sequence and functional conservation between SynTic21 and AtTic21 suggested that the TIC complex shares a similar evolutionary origin to the TOC complex.
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