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Updated: Jun 23, 2026

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
Published on: March 28, 2018
Mung bean nuclease mapping of RNAs 3' end
Daniele Bellavia1, Giorgia Sisino, Giorgio L Papadopoulos
1Dipartimento di Biologia Cellulare e dello Sviluppo - Università di Palermo - V.le delle Scienze, Edificio 16, 90128 Palermo, Italy. danielebellavia@virgilio.it
Abstract:
A method is described that allows an accurate mapping of 3' ends of RNAs. In this method a labeled DNA probe, containing the presumed 3' end of the RNA under analysis is allowed to anneals to the RNA itself. Mung-bean nuclease is then used to digest single strands of both RNA and DNA. Electrophoretic fractionation of "protected" undigested, labeled DNA is than performed using a sequence reaction of a known DNA as length marker. This procedure was applied to the analysis of both a polyA RNA (Interleukin 10 mRNA) and non polyA RNAs (sea urchin 18S and 26S rRNAs). This method might be potentially relevant for the evaluation of the role of posttrascriptional control of IL-10 in the pathogenesis of the immune and inflammatory mediated diseases associated to ageing. This might allow to develop new strategies to approach to the diagnosis and therapy of age related diseases.
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