Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
Lipid Digestion01:06

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quality control of protein import into mammalian mitochondria.

Protein science : a publication of the Protein Society·2026
Same author

ATG9A-mediated plasma membrane repair is linked to Vps13A and regulated by glycosylation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Depletion of TECPR2 leads to mitochondrial failure associated with neurodegeneration.

Autophagy·2026
Same author

A direct role for a mitochondrial targeting sequence in signalling stress.

Nature·2025
Same author

The GravyTrain toolbox for molecular cell biology.

PloS one·2025
Same author

Quality control of un-imported mitochondrial proteins at a glance.

Journal of cell science·2025

Related Experiment Video

Updated: Jun 23, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Lipophagy: selective catabolism designed for lipids.

Hilla Weidberg1, Elena Shvets, Zvulun Elazar

  • 1Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel.

Developmental Cell
|May 23, 2009
PubMed
Summary

Lipid droplet degradation was previously thought to occur in the cytosol. New research reveals that selective autophagy delivers lipid droplets for lysosomal degradation, uncovering a novel cellular pathway.

More Related Videos

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Related Experiment Videos

Last Updated: Jun 23, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Area of Science:

  • Cell Biology
  • Autophagy Research
  • Lipid Metabolism

Background:

  • Lipid droplets (LDs) are cellular organelles involved in lipid storage and metabolism.
  • Previously, the degradation of LDs was exclusively attributed to cytosolic lipases.
  • The precise mechanisms governing LD turnover remained incompletely understood.

Purpose of the Study:

  • To investigate the role of autophagy in the degradation of lipid droplets.
  • To elucidate the cellular pathways involved in lipid droplet turnover.
  • To challenge the existing paradigm of solely cytosolic lipase-mediated LD degradation.

Main Methods:

  • Utilized advanced microscopy techniques to visualize lipid droplet-autophagy interactions.
  • Employed genetic and pharmacological tools to modulate autophagic pathways.
  • Analyzed the fate of lipid droplets under conditions of impaired or enhanced autophagy.

Main Results:

  • Demonstrated the direct involvement of selective autophagy in the targeting of lipid droplets.
  • Showcased the delivery of lipid droplets to lysosomes for degradation via autophagic machinery.
  • Identified specific autophagic adaptors or receptors mediating this process (implied).

Conclusions:

  • Selective autophagy plays a crucial role in lipid droplet degradation.
  • This finding expands our understanding of cellular lipid homeostasis and metabolic regulation.
  • Challenges the long-held view of exclusive cytosolic lipase involvement in LD breakdown.