Related Concept Videos
01:21Cell Culture
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
01:16Cell Lines
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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Related Experiment Video
Updated: Jun 23, 2026
13:05
De Novo Generation of Somatic Stem Cells by YAP/TAZ
Published on: May 7, 2018
An old method for good new cells.
G Damiano1, M C Gioviale, C Lombardo
1University of Palermo School of Medicine, Palermo, Italy.
Transplantation Proceedings
|May 23, 2009
Summary
Micro-surgical in-situ perfusion significantly increased viable rat pancreatic cells by 14% compared to traditional exsanguination methods. This technique enhances cell yield for research and transplantation applications.
Area of Science:
- Regenerative Medicine
- Surgical Techniques
- Cell Biology
Background:
- Pancreatic islet isolation is crucial for diabetes research and transplantation.
- Traditional methods like exsanguination can result in lower yields of viable pancreatic cells.
- Optimizing cell retrieval is essential for improving transplantation success rates.
Purpose of the Study:
- To compare the efficacy of micro-surgical in-situ perfusion versus exsanguination for collecting viable rat pancreatic cells.
- To determine if in-situ perfusion yields a statistically significant increase in viable pancreatic cells.
Main Methods:
- Rat pancreata were collected using either micro-surgical in-situ perfusion or exsanguination (3 groups of 20 rats each).
- Perfusion involved selective cannulation of the left common iliac artery with UW solution at 4°C.
- Collected pancreata were digested, cells separated via Ficoll gradient, and cultured for viability assessment.
Main Results:
- Micro-surgical in-situ perfusion resulted in approximately 14% more viable pancreatic cells compared to exsanguination.
- The difference in viable cell yield was statistically significant (P < .001).
Conclusions:
- Micro-surgical in-situ perfusion is a superior method for obtaining viable rat pancreatic cells.
- This technique offers a significant improvement in cell yield, beneficial for pancreatic research and cell-based therapies.