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Updated: Jun 23, 2026
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
Published on: August 10, 2015
[Preparation of whole-kidney acellular matrix in rats by perfusion]
Chun-xiao Liu1, Si-ran Liu, A-bai Xu
1Department of Urological Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Liuchx888@hotmail.com
Objective:
To prepare rat whole-kidney acellular matrix (ACM) scaffolds using fluid perfusion method.
Methods:
The kidneys with ureters and renal vessels were harvested from 12-week-old Wistar rats. Intravenous catheters were inserted through the renal arteries to establish channels for whole-kidney retrograde perfusion successively with heparinized PBS, 1% SDS, deionized water, 1% TritonX-100 and antibiotic-containing PBS under a pressure of 100 cmH2O. After decellularization, the scaffolds were observed under microscope with HE staining, scanning electron microscope, and fluorescence microscope with DAPI fluorescence staining.
Results:
No cell residue was found in the scaffolds under microscope. Scanning electron microscope identified reticular structures consisting of basilar membrane and collagen without normal cellular structures in the scaffolds, and no strong fluorescence due to the binding of DAPI to the cell nuclei was observed under fluorescence microscope.
Conclusion:
Fluid perfusion is simple and reliable to prepare rat whole-kidney acellular matrix, which may serve as an ideal cell-free scaffold.
