Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Cryptococcal Meningitis

Cryptococcal Meningitis

Cryptococcal meningitis is a life-threatening opportunistic infection predominantly associated with HIV/AIDS, accounting for over 100,000 deaths annually worldwide. However, it also affects individuals with other forms of immunosuppression, including those undergoing immunosuppressive therapy, organ transplant recipients, patients with innate immunodeficiencies, and individuals with hematological disorders. The infection is caused mainly by Cryptococcus neoformans and Cryptococcus gattii,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Type 3 fimbrial regulation underpins anti-MrkA immunotherapeutic efficacy in experimental Klebsiella pneumoniae infection.

The Journal of infectious diseases·2026

Same author

Validation of molecular detection of <i>Candida auris</i> from wastewater.

Microbiology spectrum·2026

Same author

Life Identification Numbers: A strain nomenclature approach to aid epidemiological surveillance of bacterial pathogens.

PLoS biology·2026

Same author

Emergence of carbapenemase-producing Escherichia coli in acute care hospitals in 32 European countries (the CCRE survey): a prospective, multicentre, cross-sectional, epidemiological, microbiological, and genomic surveillance study.

The Lancet. Microbe·2026

Same author

Persistent spread of carbapenemase-producing Klebsiella pneumoniae in acute care hospitals in 36 European countries (the CCRE survey): a prospective, multicentre, cross-sectional, epidemiological, microbiological, and genomic surveillance study.

The Lancet. Microbe·2026

Same author

Updated Genomic Epidemiologic Description of Candida (Candidozyma) auris, United States.

Emerging infectious diseases·2026

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii.

Wieland Meyer¹, David M Aanensen, Teun Boekhout

¹Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia.

Medical Mycology

|May 23, 2009

Summary

A new multi-locus typing scheme for Cryptococcus neoformans and Cryptococcus gattii has been established for global strain genotyping. This standardized method uses seven genetic loci to improve fungal pathogen identification and tracking.

More Related Videos

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

Related Experiment Videos

Last Updated: Jun 23, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

Area of Science:

Medical Mycology
Microbial Genomics
Infectious Diseases

Background:

Cryptococcus neoformans and Cryptococcus gattii are significant fungal pathogens.
Accurate and standardized strain identification is crucial for epidemiological studies and clinical management.
Existing genotyping methods may lack global standardization.

Purpose of the Study:

To describe the consensus multi-locus typing (MLT) scheme for Cryptococcus neoformans and Cryptococcus gattii.
To establish a standardized global genotyping method for these fungal species.
To facilitate international collaboration in Cryptococcus research.

Main Methods:

Development of a consensus MLT scheme by the Cryptococcal Working Group I of ISHAM.
Selection of seven unlinked genetic loci for genotyping.
Inclusion of housekeeping genes (CAP59, GPD1, LAC1, PLB1, SOD1, URA5) and the IGS1 region.

Main Results:

A standardized seven-locus MLT scheme has been established.
The scheme allows for global strain genotyping of Cryptococcus neoformans and Cryptococcus gattii.
Allele and sequence type information is available online.

Conclusions:

The consensus MLT scheme provides a standardized approach for Cryptococcus genotyping.
This scheme will enhance global epidemiological surveillance and research on Cryptococcus neoformans and Cryptococcus gattii.
The publicly accessible database promotes data sharing and consistency.