[The effect of superoxide dismutase on histochemical detection of dehydrogenases on zymograms using sorbitol...

Sorbitol dehydrogenases from the cytoplasm of plant, animal, and microbial cells was used to study the effect of superoxide dismutase on histochemical exposure of dehydrogenases after their electrophoretic separation. In some organisms, both enzymes are localized in the same region, which finally leads to the formation of hydrasine tetrazolium, a soluble colourless compound, but not of diformazan, an insoluble stained derivative of tetrazolium nitroblue. The experimental conditions for histochemical exposure of the enzymes in gels are discussed.