Related Experiment Video
Updated: Jun 22, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Genetic engineering of antibiotic biosynthesis for the generation of new aminocoumarins
1Pharmaceutical Biology, Pharmaceutical Institute, Tübingen University, Auf der Morgenstelle 8, 72076 Tübingen, Germany.
Abstract:
The aminocoumarin antibiotics novobiocin, clorobiocin and coumermycin A(1) are inhibitors of gyrase and highly effective antibacterial agents. Their biosynthetic gene clusters have been cloned from the respective Streptomyces producer strains, and the function of nearly all genes contained therein has been elucidated by genetic and biochemical methods. Efficient methods have been developed for the genetic manipulation and the heterologous expression of the clusters, and more than 100 new derivatives of these antibiotics have been generated by metabolic engineering, mutasynthesis and chemoenzymatic synthesis, providing a model for the power of genetic and genomic methods for the generation of new bioactive compounds.
More Related Videos
10:41The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
Published on: January 13, 2013
11:45Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
Published on: May 29, 2016
Related Concept Videos
Inhibitors of Bacterial Protein Synthesis
Production of Antibiotics
Antibiotic Selection
Gene Regulation in Microbial Communities: Quorum Sensing
Production of Pharmaceuticals
Mechanism of Antibiotic Resistance in MRSA