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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Preparation and Reactions of Thiols

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Related Experiment Video

Updated: Jun 22, 2026

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Linear disulfide-containing low polymer as efficient DNA cleavage reagent.

Yong-Zhe Xiang1, Yi-Le Liao, Ji Zhang

  • 1Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR China.

Bioorganic & Medicinal Chemistry Letters
|May 26, 2009
PubMed
Summary

A novel polymer, poly[1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane] (PBMAC), enhances DNA cleavage and binding more effectively than its monomer. This macrocyclic polyamine shows promise for applications requiring efficient DNA interaction.

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Last Updated: Jun 22, 2026

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Area of Science:

  • Macromolecular Chemistry
  • Biophysical Chemistry
  • Synthetic Chemistry

Background:

  • Macrocyclic polyamines are known for their ability to interact with DNA.
  • Developing novel polymers with enhanced DNA binding and cleavage properties is of interest for various applications.

Purpose of the Study:

  • To synthesize and characterize a novel linear polymer, poly[1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane] (PBMAC).
  • To investigate the DNA cleavage and binding capabilities of PBMAC compared to its monomer, 1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane (BMAC).

Main Methods:

  • Synthesis of PBMAC via oxidation of BMAC.
  • Gel electrophoresis to assess DNA cleavage efficiency.
  • Fluorescence quenching and DNA melting experiments to evaluate DNA binding affinity.

Main Results:

  • PBMAC was successfully synthesized and characterized.
  • PBMAC demonstrated significantly enhanced DNA cleavage efficiency compared to BMAC under physiological conditions, without thiol additives.
  • PBMAC exhibited a stronger binding affinity to DNA than its monomer, as evidenced by fluorescence quenching and DNA melting studies.

Conclusions:

  • The novel linear polymer PBMAC, derived from a macrocyclic polyamine, possesses superior DNA cleavage and binding properties compared to its monomer.
  • PBMAC's enhanced performance suggests its potential utility in applications involving DNA manipulation and interaction.