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Updated: Jun 22, 2026
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Published on: November 10, 2015
Rapid detection of point mutations in Plasmodium falciparum genes associated with antimalarial drugs resistance by
Valérie Andriantsoanirina1, Vincent Lascombes, Arsène Ratsimbasoa
1Malaria Unit Research, Institut Pasteur de Madagascar, Antananarivo, Madagascar.
Abstract:
We have developed a High-Resolution DNA Melting method to detect mutations related to Plasmodium falciparum resistance. This method is based on real-time PCR followed by High Resolution Melting ramping from 67 degrees C to 80 degrees C with fluorescence data acquisition set at 0.1 degrees C increments. The accuracy of the technique was assessed using 177 P. falciparum clinical isolates and two reference strains. Results perfectly matched those obtained by DNA sequencing for some important genetic markers of P. falciparum resistance. This technique could be of great value for epidemiological studies, especially in developing countries.
