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Related Experiment Video

Updated: Jun 22, 2026

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

C/EBP{alpha} is required for pulmonary cytoprotection during hyperoxia.

Yan Xu¹, Chika Saegusa, Angelica Schehr

¹Cincinnati Children's Hospital Medical Center, University of Cincinnati, Ohio 45229-3039, USA.

American Journal of Physiology. Lung Cellular and Molecular Physiology

|May 26, 2009

Summary

CCAAT enhancer-binding protein alpha (C/EBPalpha) protects mature lungs from hyperoxic injury. Deleting C/EBPalpha in lung cells increases susceptibility to acute lung injury, highlighting its crucial protective role.

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Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

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Published on: October 19, 2013

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Last Updated: Jun 22, 2026

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

Published on: October 19, 2013

Area of Science:

Pulmonary Medicine
Molecular Biology
Cellular Biology

Background:

Transcriptional pathways regulating fetal lung development are reactivated during lung injury repair.
CCAAT enhancer-binding protein alpha (C/EBPalpha) is vital for lung maturation.

Purpose of the Study:

To investigate the role of C/EBPalpha in protecting the alveolar epithelium from hyperoxic injury in mature lungs.
To determine if C/EBPalpha is essential for maintaining lung function under acute oxidative stress.

Main Methods:

Developed transgenic Cebpalpha(Delta/Delta) mice with conditional C/EBPalpha deletion in lung cells.
Exposed mice to 95% oxygen to induce hyperoxic injury.
Analyzed lung inflammation, surfactant homeostasis, lung mechanics, and gene expression via mRNA microarrays.

Main Results:

Cebpalpha(Delta/Delta) mice exhibited high susceptibility to hyperoxia, with rapid mortality, severe inflammation, and impaired surfactant production.
Hyperoxia led to reduced expression of genes involved in surfactant homeostasis (e.g., Srebf, Abca3, Sftpb) in Cebpalpha(Delta/Delta) mice.
Genes related to cell signaling and immune responses were upregulated, while those for redox homeostasis were downregulated in knockout mice.

Conclusions:

C/EBPalpha is indispensable for regulating genes critical for surfactant homeostasis, protein processing, and cellular defense during hyperoxic lung injury.
While not essential for normal lung function, C/EBPalpha mediates vital protection against acute lung injury induced by hyperoxia.