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Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants
Maria Jönsson1, Zhian Saleihan, Ingolf F Nes
1Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 As, Norway. maria.jonsson@umb.no
Applied and Environmental Microbiology
|May 26, 2009
Abstract:
The roles of the two ldh genes of Enterococcus faecalis were studied using knockout mutants. Deletion of ldh-1 causes a metabolic shift from homolactic fermentation to ethanol, formate, and acetoin production, with a high level of formate production even under aerobic conditions. Ldh-2 plays only a minor role in lactate production.
