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Related Experiment Video

Updated: Jun 22, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Practical implementation of adaptive optics in multiphoton microscopy.

P Marsh, D Burns, J Girkin

    Optics Express
    |May 26, 2009
    PubMed
    Summary
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    This study introduces an adaptive-optic two-photon microscope that corrects aberrations, significantly extending imaging depth in biological samples. The new system enhances deep-tissue imaging capabilities for microscopy applications.

    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Optical Engineering

    Background:

    • Specimen-induced aberrations limit imaging depth in two-photon microscopy.
    • Adaptive optics offer a potential solution to overcome these limitations.

    Purpose of the Study:

    • To develop and evaluate a compact adaptive-optic module for two-photon microscopy.
    • To demonstrate enhanced imaging depth and resolution in refractive-index mismatched samples.

    Main Methods:

    • A dedicated two-photon microscope was built with a micromachined deformable mirror for wavefront correction.
    • The adaptive optic system utilized multiphoton fluorescence intensity feedback for automatic aberration correction.
    • Imaging depth and resolution were tested in artificial and biological samples using a high-NA oil immersion objective.

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    Last Updated: Jun 22, 2026

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    Bringing the Visible Universe into Focus with Robo-AO
    10:35

    Bringing the Visible Universe into Focus with Robo-AO

    Published on: February 12, 2013

    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    Main Results:

    • The adaptive optic system successfully corrected specimen-induced aberrations.
    • Imaging depth in water was extended from 3.4 to 46.2 micrometers.
    • Resolution was maintained with a full width at half maximum (FWHM) axial point-spread function of 1.25 micrometers.

    Conclusions:

    • The developed adaptive-optic two-photon microscope significantly extends imaging depth.
    • This technology improves deep-tissue imaging in scattering biological samples.
    • The compact design is compatible with existing microscopy systems.