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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 22, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Multiphoton plasmon-resonance microscopy.

Dvir Yelin, Dan Oron, Stephan Thiberge

|May 26, 2009

Summary

This summary is machine-generated.

A new method uses noble-metal nanoparticles

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Last Updated: Jun 22, 2026

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Area of Science:

Biophysics
Nanotechnology
Cell Biology

Background:

Noble-metal nanoparticles exhibit unique nonlinear optical properties.
Specific labeling of cellular organelles is crucial for biological research.
Nonlinear optical microscopy offers high resolution and depth-specificity.

Purpose of the Study:

To present a novel method for detecting noble-metal nanoparticles using their nonlinear optical properties.
To apply this method for specific labeling of cellular organelles.
To demonstrate depth-resolved imaging of labeled cellular structures.

Main Methods:

Illumination of noble-metal nanoparticles with laser light at their plasmon frequency.
Measurement of the enhanced multiphoton signal generated by the nanoparticles.
Utilizing a laser scanning microscope setup for imaging.
Employing two-photon autofluorescence or third-harmonic generation for signal detection.

Main Results:

Successful detection of noble-metal nanoparticles based on their nonlinear optical response.
Specific labeling of cellular organelles and membranes in both live and fixed cells.
Generation of depth-resolved images with high spatial resolution.
Demonstration of the technique's versatility through different nonlinear optical signals.

Conclusions:

The presented method enables sensitive and specific detection of noble-metal nanoparticles.
This technique provides a powerful tool for labeling and imaging cellular organelles.
Nonlinear optical properties of nanoparticles offer a promising avenue for advanced bioimaging applications.