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Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

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Published on: May 11, 2018

Second harmonic microscopy of axonemes.

Christophe Odin¹, Claire Heichette, Denis Chretien

¹Institut of Physics of Rennes IPR/UMR CNRS 6251, University of Rennes I, Campus de Beaulieu, Bat 11A, 35042 Rennes Cedex, France. christophe.odin@univ-rennes1.fr

|May 26, 2009

Summary

Researchers used Second Harmonic Microscopy to analyze sea urchin sperm axonemes. They determined the nonlinear susceptibility of axonemes, providing new insights into their optical properties.

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Published on: February 11, 2022

Area of Science:

Biophysics
Microscopy

Background:

Axonemes are crucial microtubule-based structures responsible for motility.
Understanding their optical properties can reveal insights into their structural organization and function.

Purpose of the Study:

To determine the nonlinear susceptibility of sea urchin sperm axonemes.
To characterize the optical properties of axonemes using advanced microscopy techniques.

Main Methods:

Second Harmonic Microscopy was employed on sea urchin sperm axonemes.
Polarization analysis was utilized to optimize signal and minimize photodamage.

Main Results:

The nonlinear susceptibility ratios chizxx/chixzx = 1.1+/-0.2 and chizzz/chixzx = 4+/-0.5 were determined.
This marks the first reported measurement of these specific nonlinear optical properties for axonemes.

Conclusions:

The study provides novel quantitative data on the nonlinear optical properties of axonemes.
These findings contribute to a deeper understanding of axoneme structure and potential applications in biophotonics.