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Updated: May 27, 2026

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway

Weifeng Ding1, Shaoqing Ju, Shengyang Jiang

  • 1Medical Laboratory Center, Affiliated Hospital of Nantong University, No.20, Road Xisi, Nantong, China.

Pathology Oncology Research : POR
|May 26, 2009
PubMed

Insights

Decreased levels of APRIL (a proliferation-inducing protein) in colon cancer cells led to senescence, a process involving HSPG. This suggests APRIL

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • APRIL, a TNF superfamily member, promotes cell proliferation and is often overexpressed in tumors.
  • The impact of reduced APRIL expression on tumor cells remains largely unexplored.

Purpose of the Study:

  • To investigate the effects of decreased APRIL expression on colon carcinoma cell line SW480.
  • To explore the role of HSPG in the context of APRIL modulation in these cells.

Main Methods:

  • Western blot and RT-PCR were used to analyze APRIL and HSPG expression.
  • APRIL levels were experimentally reduced (knockdown) in SW480 cells.

Main Results:

  • Both APRIL and HSPG expression were found to be upregulated in SW480 cells.
  • APRIL knockdown significantly reversed cell proliferation and induced partial senescence phenotypes.
  • Cellular senescence observed was linked to HSPG engagement.

Conclusions:

  • Reduced APRIL levels are crucial for inducing cellular senescence in SW480 cells.
  • This senescence process is mediated through a HSPG-dependent signaling pathway.

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