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Updated: May 27, 2026
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Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway
Weifeng Ding1, Shaoqing Ju, Shengyang Jiang
1Medical Laboratory Center, Affiliated Hospital of Nantong University, No.20, Road Xisi, Nantong, China.
Pathology Oncology Research : POR
|May 26, 2009
Summary
Decreased levels of APRIL (a proliferation-inducing protein) in colon cancer cells led to senescence, a process involving HSPG. This suggests APRIL
Area of Science:
- Oncology
- Cell Biology
- Molecular Biology
Background:
- APRIL, a TNF superfamily member, promotes cell proliferation and is often overexpressed in tumors.
- The impact of reduced APRIL expression on tumor cells remains largely unexplored.
Purpose of the Study:
- To investigate the effects of decreased APRIL expression on colon carcinoma cell line SW480.
- To explore the role of HSPG in the context of APRIL modulation in these cells.
Main Methods:
- Western blot and RT-PCR were used to analyze APRIL and HSPG expression.
- APRIL levels were experimentally reduced (knockdown) in SW480 cells.
Main Results:
- Both APRIL and HSPG expression were found to be upregulated in SW480 cells.
- APRIL knockdown significantly reversed cell proliferation and induced partial senescence phenotypes.
- Cellular senescence observed was linked to HSPG engagement.
Conclusions:
- Reduced APRIL levels are crucial for inducing cellular senescence in SW480 cells.
- This senescence process is mediated through a HSPG-dependent signaling pathway.
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