Evaluating the potential eye irritancy of shampoos
M Bell1, P M Holmes, T M Nisbet
1Medical Affairs and Safety Evaluation Unit, Beecham Products Research Department, Randalls Road, Leatherhead, Surrey, England.
International Journal of Cosmetic Science
|May 27, 2009
Summary
Shampoo splashes rarely cause serious eye irritation in humans. Standardized rabbit eye tests at 2.5-3.0% active matter are recommended for predicting new shampoo irritancy.
Area of Science:
- Ophthalmology
- Dermatology
- Toxicology
Background:
- Industrial experience indicates minimal eye irritation from shampoo splashes.
- Consumer complaints regarding shampoo eye irritation are rare and non-severe.
- Predictive testing is crucial for new shampoo formulations to ensure safety.
Purpose of the Study:
- To evaluate the safety of new shampoo formulations.
- To establish reliable methods for predicting shampoo eye irritancy.
- To refine rabbit eye testing protocols for accurate human response prediction.
Main Methods:
- Analysis of industrial incident data and consumer complaints.
- Rabbit eye testing at specific detergent concentrations (2.5-3.0% active matter).
- In vitro screening using human buccal mucosa cells.
Main Results:
- Shampoo splashes caused only transient eye effects in manufacturing staff.
- Rabbit eye tests at 2.5-3.0% active matter effectively discriminate formulations without causing animal distress.
- In vitro tests show potential for preliminary irritancy screening.
Conclusions:
- Rabbit eye testing at 2.5-3.0% active matter is recommended for typical anionic shampoos.
- Higher concentrations in rabbit eye tests should be used cautiously, only for new ingredients.
- Comparison with a known well-tolerated control formulation is advised for rabbit eye tests.
