Related Experiment Video
Updated: Jun 22, 2026

12:33
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Published on: November 15, 2013
[Not Available].
1Laboratoire de Recherche de l'Oréal, 1 Avenue de Saint-Germain, 93600 - Aulnay sous Bois, France.
International Journal of Cosmetic Science
|May 27, 2009
Summary
This study compares three methods for quantitatively measuring human surface lipids. The adapted Schaeffer and Kuhn-Bussius method proved quantitative and correlated with skin types.
Area of Science:
- Dermatology
- Biophysics
Background:
- Accurate quantification of human surface lipids is crucial for understanding skin physiology.
- Existing methods for surface lipid measurement vary in practicability and data output.
Purpose of the Study:
- To compare the information and routine practicability of three quantitative methods for measuring surface lipids in humans.
- To adapt and standardize a specific method for reliable surface lipid assessment.
Main Methods:
- Comparison of three established quantitative methods for surface lipid measurement.
- Adaptation and standardization of the Schaeffer and Kuhn-Bussius method using a photoelectric absorptiometer and ground glass plates.
- Application of the standardized method to thirty-two adult subjects.
Main Results:
- The adapted Schaeffer and Kuhn-Bussius method demonstrated a quantitative sampling mechanism.
- A mathematical approach provided a precise definition of the 'casual-level' of surface lipids.
- Casual-level values were found to be correlated with different skin types.
Conclusions:
- The adapted Schaeffer and Kuhn-Bussius method offers a reliable and quantitative approach to measuring surface lipids.
- The 'casual-level' of surface lipids is a quantifiable parameter that can be correlated with individual skin types.