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Updated: Jun 22, 2026
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Published on: August 27, 2014
Kinetic study of the cetyltrimethylammonium/DNA interaction
E Grueso1, E Roldan, F Sanchez
1The Department of Physical Chemistry, University of Seville, C/Profesor Garcia Gonzalez s/n, 41012 Seville, Spain.
Abstract:
A kinetic study of the interaction of the surfactant cetyltrimethylammonium (CTA(+)) with DNA was carried out in water and in salt (NaCl) solutions. The results can be explained in terms of a reaction mechanism involving two consecutive reversible steps. The first step corresponds to the union/separation of the surfactant with/from the DNA. The second step corresponds to a conformational change of the surfactant/DNA complex. The equilibrium constant, calculated from the forward and reverse rate constants of these steps, agrees with the results of a previous thermodynamic study.
