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Related Experiment Video

Updated: Jun 22, 2026

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
11:56

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

Published on: June 16, 2017

Drosophila multiplexin (Dmp) modulates motor axon pathfinding accuracy.

Frauke Meyer1, Bernard Moussian

  • 1Max-Planck-Institute for Developmental Biology, Department III - Genetics, Spemannstrasse 35, 72076 Tübingen, Germany.

Development, Growth & Differentiation
|May 28, 2009
PubMed
Summary

Drosophila multiplexin (dmp) influences motor axon pathfinding. Monomeric endostatin rescues defects, while trimeric endostatin enhances accuracy, suggesting a role in preventing innervation errors.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Neuroscience

Background:

  • Multiplexins are multidomain collagens with regulatory roles in tissue development, primarily via their endostatin domain.
  • The endostatin domain exists in monomeric and trimeric forms, exhibiting opposing effects on cell migration.
  • The single Drosophila multiplexin (dmp) gene encodes isoforms with features of vertebrate collagens XV and XVIII.

Purpose of the Study:

  • To characterize the Drosophila multiplexin (dmp) gene and its isoforms.
  • To investigate the role of Dmp in motor axon pathfinding during Drosophila development.
  • To determine the specific functions of different Dmp isoforms in axon guidance.

Main Methods:

  • Gene characterization and isoform identification of Drosophila multiplexin (dmp).

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Last Updated: Jun 22, 2026

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
11:56

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

Published on: June 16, 2017

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
08:25

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Published on: January 12, 2020

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics
09:42

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Published on: November 7, 2011

  • Generation of dmp deletion alleles to create mutant models.
  • Transgenic overexpression of Dmp isoforms to assess rescue and enhancement effects on axon pathfinding.

    • Main Results:

    • Three main dmp isoforms were identified, including a monomeric endostatin version.
    • dmp deletion mutants displayed motor axon pathfinding defects, specifically ventral bypass defects in the intersegmental nerve b (ISNb).
    • Overexpression of monomeric endostatin and full-length Dmp rescued these defects, while trimeric endostatin improved accuracy in wild-type backgrounds.

    Conclusions:

    • Drosophila multiplexin (dmp) plays a crucial role in modulating motor axon pathfinding.
    • Different Dmp isoforms (monomeric vs. trimeric endostatin) have distinct functions in axon guidance.
    • Dmp may function within a buffering system to prevent motor innervation errors.