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Updated: Jun 22, 2026
05:32
A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device
Published on: November 24, 2016
A new approach to assessing sweat production
1138 Jarrom Street, Leicester LE2 7DF, U.K.
International Journal of Cosmetic Science
|May 28, 2009
Summary
Researchers investigated antiperspirant mechanisms using isolated rat sweat glands. They found that sweat production stopped with atropine or calcium ion removal, offering a new model for antiperspirant research.
Area of Science:
- Physiology
- Dermatology
- Pharmacology
Background:
- Excessive sweating (hyperhidrosis) affects many individuals, impacting quality of life.
- Antiperspirants, particularly aluminum chloride, have been used since 1916 to reduce sweating.
- The precise mechanism of action for antiperspirants remains incompletely understood, necessitating further research.
Purpose of the Study:
- To investigate the mechanism of action of antiperspirants.
- To evaluate a novel in vitro model using isolated rat sweat glands for studying sweat production.
- To determine the effects of specific agents on sweat gland function.
Main Methods:
- Isolated rat plantar sweat glands were utilized as a model system.
- Sweat production was stimulated using carbachol and methacholine.
- Glands were maintained in a controlled physiological environment (modified Krebs-Ringer bicarbonate at 38°C with rat serum).
- Sweat output was measured over 85 minutes, with maximal volumes recorded.
- The effects of atropine and calcium ion concentration on sweat production were assessed.
Main Results:
- The isolated rat sweat gland model successfully produced measurable sweat output.
- Maximal sweat volume obtained from individual glands was 23 nanolitres.
- Sweat production was effectively inhibited by the administration of atropine.
- Cessation of sweat production was also observed upon withdrawal of calcium ions from the bathing medium.
Conclusions:
- The isolated rat sweat gland model provides a viable system for studying sweat production and antiperspirant mechanisms, free from systemic influences like blood flow.
- Atropine and calcium ions play critical roles in regulating sweat gland secretion.
- This model holds promise for future evaluations of antiperspirant efficacy and mode of action.
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