Related Experiment Video
Updated: Jun 22, 2026
07:51
Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
Published on: June 10, 2017
Multiple perturbation two-dimensional correlation analysis of cellulose by attenuated total reflection infrared
Hideyuki Shinzawa1, Shin-Ich Morita, Kimie Awa
1Optical Biopsy Development Research Unit, RIKEN, Saitama, Japan. hshinzawa@riken.jp
Applied Spectroscopy
|May 28, 2009
Summary
This study introduces a new multi-dimensional analysis for microscopic imaging data. It reveals how cellulose crystallinity affects water evaporation rates during drying processes.
Area of Science:
- Spectroscopy and Analytical Chemistry
- Materials Science
- Physical Chemistry
Background:
- Two-dimensional (2D) correlation analysis is a powerful tool for analyzing complex spectral data.
- Analyzing systems with multiple independent perturbations, such as material properties and environmental conditions, presents significant challenges.
- Understanding the interplay between material structure and dynamic processes is crucial in various scientific fields.
Purpose of the Study:
- To extend the two-dimensional (2D) correlation analysis scheme to handle multi-dimensional perturbations.
- To develop a computational method for constructing synchronous correlation and disrelation maps for microscopic imaging data.
- To investigate the complex behavior of water and cellulose mixtures during water evaporation under varying grinding times.
Main Methods:
- Development of an extended two-dimensional (2D) correlation analysis for multi-dimensional perturbation.
- Implementation of a computational form for synchronous correlation and disrelation map construction.
- Acquisition of time-dependent attenuated total reflection infrared (ATR-IR) spectra from water and cellulose mixtures during water evaporation.
Main Results:
- The multiple perturbation 2D analysis successfully identified complex behaviors in the water-cellulose system.
- A specific difference in the rate of water molecule evaporation was observed in relation to changes in cellulose crystallinity.
- Subtle differences in water volatility were detected and linked to the crystalline structure of cellulose.
Conclusions:
- The developed multi-dimensional 2D correlation analysis is effective for complex systems with multiple perturbations.
- Cellulose crystallinity significantly influences the evaporation rate of water molecules.
- The study highlights the relationship between water volatility and the structural properties of cellulose.
More Related Videos
09:27High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
Published on: May 10, 2016
10:46Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
Published on: March 12, 2010
Related Concept Videos
01:13Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
The ATR process begins by directing a beam...
01:16IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to the...
According to Hooke's law, the vibrational frequency is directly proportional to the...
01:28IR and UV–Vis Spectroscopy of Carboxylic Acids
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
01:24IR Spectroscopy: Molecular Vibration Overview
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
01:20Raman Spectroscopy: Overview
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
01:11Applications of IR Spectroscopy: Overview
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...