Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental sepsis causes SERCA2 expression in white adipose tissue but not classical browning.

Scientific reports·2026
Same author

Targeting lysosomal pH restores mitochondrial quality control in GBA1-mutant Parkinson's disease.

Translational neurodegeneration·2026
Same author

Mitophagy: an emerging therapeutic target in mitochondrial diseases.

The Biochemical journal·2026
Same author

First-in-human to proof-of-concept: why experimental medicine studies remain essential in human physiology and drug development.

Pflugers Archiv : European journal of physiology·2026
Same author

Pathogenic variants in the autophagy-tethering factor EPG5 drive neurodegeneration through mitochondrial dysfunction and innate immune activation.

Nature communications·2026
Same author

Distinct proteomic signatures of urinary extracellular vesicles link to albuminuria and treatment responses in diabetic kidney disease.

Kidney international·2026

Related Experiment Video

Updated: Jun 22, 2026

Assessment of Mitochondrial Fission/Fusion Dynamics in Kidney Proximal Tubular Cells
06:14

Assessment of Mitochondrial Fission/Fusion Dynamics in Kidney Proximal Tubular Cells

Published on: November 14, 2025

Multiphoton imaging reveals differences in mitochondrial function between nephron segments.

Andrew M Hall1, Robert J Unwin, Nadeene Parker

  • 1Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK. andrew.hall@ucl.ac.uk

Journal of the American Society of Nephrology : JASN
|May 28, 2009
PubMed
Summary
This summary is machine-generated.

Mitochondria in kidney proximal tubules exhibit higher oxidative stress and lower membrane potential compared to distal tubules. These differences in mitochondrial function may explain kidney injury susceptibility in specific nephron segments.

More Related Videos

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
10:31

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Published on: September 29, 2017

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
07:32

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Published on: February 9, 2020

Related Experiment Videos

Last Updated: Jun 22, 2026

Assessment of Mitochondrial Fission/Fusion Dynamics in Kidney Proximal Tubular Cells
06:14

Assessment of Mitochondrial Fission/Fusion Dynamics in Kidney Proximal Tubular Cells

Published on: November 14, 2025

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
10:31

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Published on: September 29, 2017

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
07:32

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Published on: February 9, 2020

Area of Science:

  • Nephrology
  • Mitochondrial Biology
  • Renal Pathophysiology

Background:

  • Mitochondrial dysfunction is implicated in renal disease pathogenesis.
  • Significant differences exist in metabolic demands across nephron tubule segments.
  • The proximal tubule is particularly susceptible to mitochondrial toxicity.

Purpose of the Study:

  • To investigate axial differences in mitochondrial function along the nephron.
  • To compare mitochondrial properties between proximal and distal tubules.
  • To elucidate mechanisms underlying differential mitochondrial vulnerability in the kidney.

Main Methods:

  • Multiphoton imaging of live rat kidney slices.
  • Assessment of mitochondrial membrane potential and respiration.
  • Measurement of reactive oxygen species (ROS) production and glutathione levels.
  • Immunostaining for mitochondrial F1F(o)-ATPase and its inhibitor IF1.

Main Results:

  • Mitochondrial membrane potential was significantly higher in distal tubules compared to proximal tubules.
  • Proximal tubule mitochondria showed rapid membrane potential collapse upon respiratory inhibition, unlike distal tubules.
  • ATPase activity more effectively maintained membrane potential in distal tubules.
  • Proximal tubules exhibited higher ROS production and glutathione levels, indicating a more oxidized mitochondrial state.
  • The ratio of ATPase to IF1 expression was lower in proximal tubules.

Conclusions:

  • Significant axial differences in mitochondrial function exist along the nephron.
  • Proximal tubule mitochondria are more susceptible to dysfunction and oxidative stress.
  • These functional disparities may underlie the specific patterns of renal injury observed in different nephron segments.