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Related Concept Videos

Plant Hormones01:56

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
Plant Hormones01:56

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Chemical Signaling in the Endocrine System01:08

Chemical Signaling in the Endocrine System

A signaling cascade is a series of events that facilitates the transmission of information within or between cells, culminating in a targeted response in the recipient cell. As chemical messengers, hormones are pivotal in initiating and modulating these intricate signaling cascades based on their solubility.
Lipid-soluble hormones, such as steroid hormones, demonstrate an intracellular action. These hormones traverse cell membranes due to their lipid nature. Once inside the target cell, they...
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
Types of Hormones02:13

Types of Hormones

Hormones can be classified into three main types based on their chemical structures: steroids, peptides, and amines. Their actions are mediated by the specific receptors they bind to on target cells.

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Updated: Jun 22, 2026

Monitoring Plant Hormones During Stress Responses
11:01

Monitoring Plant Hormones During Stress Responses

Published on: June 15, 2009

Phytohormones as important biologically active molecules--their simple simultaneous detection.

Vaclav Diopan1, Vojtech Adam, Ladislav Havel

  • 1Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic.

Molecules (Basel, Switzerland)
|May 28, 2009
PubMed
Summary
This summary is machine-generated.

This study optimized liquid chromatography methods for accurately detecting plant hormones and their metabolites in plant tissues. The developed method achieved sensitive detection limits, crucial for plant science research.

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Area of Science:

  • Plant Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Phytohormones are critical for plant growth and development.
  • Accurate and sensitive methods are needed to study phytohormones and their metabolites in plant tissues.

Purpose of the Study:

  • To optimize experimental conditions for separating and determining plant hormones and their metabolites.
  • To establish sensitive detection limits for various phytohormones and their derivatives.
  • To validate the method's applicability using plant extracts and a commercial product.

Main Methods:

  • Liquid chromatography coupled with a UV-VIS detector was employed.
  • Optimization of chromatographic conditions including column, mobile phase, flow rate, temperature, and wavelength.
  • Analysis of a mixture containing various cytokinins, auxins, and their metabolites.
  • Method validation through calibration, limit of detection, and quantification estimation.

Main Results:

  • Optimal chromatographic conditions were established using a Polaris C18 column and a specific gradient mobile phase.
  • Detection limits for target molecules were in the tens of ng/mL range.
  • Recovery rates for cytokinins, their metabolites, and auxins ranged from 87% to 105% in flax extracts.
  • The method accurately quantified alpha-naphthalene acetic acid in a commercial root formation stimulant.

Conclusions:

  • The optimized liquid chromatography method provides accurate and sensitive quantification of plant hormones and their metabolites.
  • This method is suitable for analyzing complex plant samples and commercial formulations.
  • The findings contribute to advancing phytohormone research in plant science.