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Related Experiment Video

Updated: Jun 22, 2026

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

Comparison between L and E gene amplification analytical methods for human papillomavirus typing.

Hong-Bum Cho1, Young-Jae Kim, Kyung-Tai Kim

  • 1Department of Biological Engineering, SeoKyeong University, Seoul, Korea.

Journal of Gynecologic Oncology
|May 28, 2009
PubMed
Summary

Comparing L1 and E6/E7 gene amplification methods for human papillomavirus (HPV) typing revealed significant differences. Parallel amplification of both L1 and E6/E7 genes improves diagnostic accuracy for HPV infections.

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Area of Science:

  • Virology
  • Molecular Biology
  • Medical Diagnostics

Background:

  • Accurate human papillomavirus (HPV) typing is crucial for risk stratification and patient management.
  • Existing HPV typing methods may have limitations in sensitivity and specificity.

Purpose of the Study:

  • To compare the efficacy of L1 gene and E6/E7 gene amplification analyses for HPV detection and typing.
  • To minimize HPV typing errors by evaluating different molecular approaches.

Main Methods:

  • HPV typing using L1 gene amplification via polymerase chain reaction (PCR) and membrane assays.
  • HPV typing verification using E6/E7 gene amplification via nested multiplexed PCR.
  • Comparative analysis of results from 104 patient samples.
Keywords:
E6/E7 geneHuman papillomavirusL1 gene

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Use of Interferon-&gamma; Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

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Last Updated: Jun 22, 2026

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

Use of Interferon-&gamma; Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Main Results:

  • A 35% rate of accordance and a 65% rate of difference were observed between the two methods.
  • Nested multiplexed PCR demonstrated greater diversity in HPV type identification for 29% of the samples compared to membrane assays.

Conclusions:

  • HPV classification into low-risk and high-risk groups is essential for clinical decision-making.
  • Parallel amplification of both L1 and E6/E7 genes offers a more efficient and accurate diagnostic approach for HPV infections, considering the distinct clinical implications of different risk groups.