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Updated: Jun 22, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

[Methicillin resistant Staphylococcus aureus: phenotypic and genotypic studies].

M Jemili-Ben Jomaa¹, I Boutiba-Ben Boubaker, Saïda Ben Redjeb

¹Laboratoire de recherche Résistances aux antibiotiques, Faculté de Médecine de Tunis.

La Tunisie Medicale

|May 29, 2009

Summary

This study investigated Methicillin-Resistant Staphylococcus aureus (MRSA) epidemiology. Phenotypic methods combined with Random Amplified Polymorphic DNA (RAPD) fingerprinting effectively typed MRSA strains.

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Last Updated: Jun 22, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Area of Science:

Microbiology
Epidemiology
Molecular Biology

Context:

Staphylococcus aureus is a significant human opportunistic pathogen.
Increasing rates of methicillin resistance contribute to morbidity and mortality.
Understanding MRSA epidemiology is crucial for infection control.

Purpose:

To evaluate the epidemiologic situation of Methicillin-Resistant Staphylococcus aureus (MRSA) at Charles Nicolle hospital.
To assess the utility of phenotypic methods and RAPD fingerprinting for MRSA typing.

Summary:

A four-year retrospective study identified 65 MRSA isolates.
Methicillin resistance was confirmed via mec A PCR.
Random Amplified Polymorphic DNA (RAPD) using ERIC-IR revealed 7 genotypes despite biotypic similarity.

Impact:

Phenotypic methods and RAPD fingerprinting offer a routine approach for MRSA typing.
Further investigation into the phylogenetic relationships of MRSA strains is warranted.
This research contributes to the understanding of MRSA epidemiology and typing methodologies.