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Updated: Jun 22, 2026
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Published on: July 27, 2021
[Construction of plasmid expression vector for specific peptide of the rubella virus E1 gene]
Jing Cao1, Yu-Feng Huang, Jian Gao
1Department of Immunology, School of Preclinical Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Objective:
To construct a recombinant plasmid vector of the RV specific fragment for expressing the specific fragment of RV E1 protein.
Methods:
RNA of the RV attenuated live vaccine Wistar RA27/3 strain was extracted and reversely transcribed. The specific fragment of the E1 gene was amplified and the PCR products cloned in the vector pGEX-2T after purification. Positive clones were selected and identified by two-enzyme digestion and sequence analysis.
Results:
A 330 bp target fragment was successfully cloned, and the sequence of the recombinant plasmid was consistent with the original sequence.
Conclusion:
Successful cloning of the RV El specific fragment and the construction of the recombinant plasmid have laid a foundation for further expressing the recombinant protein.
